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Addgene

D282N Cus2p
(Plasmid #73060)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73060 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p11
  • Backbone manufacturer
    Midwest Center for Structural Genomics
  • Backbone size w/o insert (bp) 5704
  • Total vector size (bp) 6538
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CUS2p
  • Alt name
    U2 snRNP complex subunit CUS2
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    858
  • Mutation
    Asp 282 to Asn
  • GenBank ID
    855430
  • Entrez Gene
    CUS2 (a.k.a. YNL286W)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x His tag fused to TEV cleavage site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
  • 3′ sequencing primer TGGATGCTGTAGGCATAGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D282N Cus2p was a gift from Aaron Hoskins (Addgene plasmid # 73060 ; http://n2t.net/addgene:73060 ; RRID:Addgene_73060)

  • For your References section:

    Conformational dynamics of stem II of the U2 snRNA. Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins AA. RNA. 2015 Dec 2. 10.1261/rna.052233.115 PubMed 26631165
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