L284F Cus2p
(Plasmid
#73059)
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PurposeS. cerevisiae Cus2p gene with L284F Mutation
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep11
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Backbone manufacturerMidwest Center for Structural Genomics
- Backbone size w/o insert (bp) 5704
- Total vector size (bp) 6538
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCUS2p
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Alt nameU2 snRNP complex subunit CUS2
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)858
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MutationLeucine 284 to Phenylalanine
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GenBank ID855430
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Entrez GeneCUS2 (a.k.a. YNL286W)
- Promoter T7
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Tag
/ Fusion Protein
- 6x His tag fused to TEV cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer TAA TAC GAC TCA CTA TAG GG
- 3′ sequencing primer TGGATGCTGTAGGCATAGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L284F Cus2p was a gift from Aaron Hoskins (Addgene plasmid # 73059 ; http://n2t.net/addgene:73059 ; RRID:Addgene_73059) -
For your References section:
Conformational dynamics of stem II of the U2 snRNA. Rodgers ML, Tretbar US, Dehaven A, Alwan AA, Luo G, Mast HM, Hoskins AA. RNA. 2015 Dec 2. 10.1261/rna.052233.115 PubMed 26631165