pCIG AKT3 E17K
(Plasmid
#73050)
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PurposeHuman AKT3 E17K
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAKT3 E17K
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SpeciesH. sapiens (human)
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Insert Size (bp)7647
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MutationE17K
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Entrez GeneAKT3 (a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ggcgtgtgaccggcggctctagagcc (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIG AKT3 E17K was a gift from Joseph Gleeson (Addgene plasmid # 73050 ; http://n2t.net/addgene:73050 ; RRID:Addgene_73050) -
For your References section:
An AKT3-FOXG1-reelin network underlies defective migration in human focal malformations of cortical development. Baek ST, Copeland B, Yun EJ, Kwon SK, Guemez-Gamboa A, Schaffer AE, Kim S, Kang HC, Song S, Mathern GW, Gleeson JG. Nat Med. 2015 Dec;21(12):1445-54. doi: 10.1038/nm.3982. Epub 2015 Nov 2. 10.1038/nm.3982 PubMed 26523971