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Addgene

pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
(Plasmid #73040)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73040 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS
  • Backbone manufacturer
    Systembio
  • Backbone size w/o insert (bp) 6396
  • Total vector size (bp) 8387
  • Modifications to backbone
    ClaI/SalI fragment was replaced with EF1s + new MCS
  • Vector type
    Mammalian Expression, Lentiviral, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nluc-P2A-copGFP-T2A-Puro
  • Species
    Synthetic
  • Insert Size (bp)
    2019
  • Promoter PGK (phosphoglycerate kinase 1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GCCAATAGCAGCTTTGCTCC
  • 3′ sequencing primer CAACACCACGGAATTGTCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 73040 ; http://n2t.net/addgene:73040 ; RRID:Addgene_73040)
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