pLJM1 RagA
(Plasmid #73031)

• Purpose: Expresses human RagA from a CMV promoter for stable expression in mammalian cells
• Depositing Lab: David Sabatini
• Publication: Efeyan et al Dev Cell. 2014 May 12;29(3):321-9. doi: 10.1016/j.devcel.2014.03.017. Epub 2014 Apr 24.

Backbone

• Vector backbone: pLJM1
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 9000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rragA
• Alt name: RagA
• Species: H. sapiens (human)
• Insert Size (bp): 950
• Entrez Gene: RRAGA (a.k.a. FIP-1, FIP1, RAGA)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: pLJM1 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned from pRK5-RagA from the Sabatini lab
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry