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Addgene

pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant
(Plasmid #72924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN_TTmiRC2
  • Backbone manufacturer
    Addgene#25752
  • Backbone size w/o insert (bp) 4422
  • Total vector size (bp) 5695
  • Vector type
    Bacterial Expression ; entry vector for gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GATA Binding Protein 6
  • Alt name
    GATA6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1917
  • Mutation
    Serine#37 changed to Alanine;Silent mutations made on Arginine#479,Proline#480,Leucine#481 & Alanine#482
  • GenBank ID
    NM_005257
  • Entrez Gene
    GATA6
  • Promoter TRE tight
  • Tags / Fusion Proteins
    • 3XFLAG (N terminal on insert)
    • 3XAU1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer pCEP-fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant was a gift from Kevin Janes (Addgene plasmid # 72924 ; http://n2t.net/addgene:72924 ; RRID:Addgene_72924)

  • For your References section:

    TNF-insulin crosstalk at the transcription factor GATA6 is revealed by a model that links signaling and transcriptomic data tensors. Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger DA, Janes KA. Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373. 10.1126/scisignal.aad3373 PubMed 27273097
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