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dmBACCS2VL-IRES-dOrai-IRES-GFP
(Plasmid #72897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWPRE/C1 (modified pEGFP-C1)
  • Backbone manufacturer
    Modified from Clontech
  • Backbone size w/o insert (bp) 4581
  • Total vector size (bp) 9127
  • Modifications to backbone
    The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was inserted into the BamHI site of pEGFP-C1, and EGFP was removed.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dmBACCS2VL-IRES-dOrai-IRES-GFP
  • Species
    D. melanogaster (fly); Avena sativa (oat)
  • Insert Size (bp)
    4546
  • Promoter CMV
  • Tag / Fusion Protein
    • IRES-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

dmBACCS2VL is a slow recovery kinetic mutant of dmBACCS2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dmBACCS2VL-IRES-dOrai-IRES-GFP was a gift from Takao Nakata (Addgene plasmid # 72897 ; http://n2t.net/addgene:72897 ; RRID:Addgene_72897)

  • For your References section:

    Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Ishii T, Sato K, Kakumoto T, Miura S, Touhara K, Takeuchi S, Nakata T. Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021. 10.1038/ncomms9021 PubMed 26282514
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