dmBACCS2NS-IRES-dOrai-IRES-mCherry
(Plasmid #72896)

• Purpose: Mammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and mCherry
• Depositing Lab: Takao Nakata
• Publication: Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.1038/ncomms9021.

Backbone

• Vector backbone: pWPRE/C1 (modified pEGFP-C1)
• Backbone manufacturer: Modified from Clontech
• Backbone size w/o insert (bp): 4581
• Total vector size (bp): 9118
• Modifications to backbone: The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) was inserted into the BamHI site of pEGFP-C1, and EGFP was removed.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dmBACCS2NS-IRES-dOrai-IRES-mCherry
• Species: D. melanogaster (fly); Avena sativa (oat)
• Insert Size (bp): 4537
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES-mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: WPRE-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

dmBACCS2NS is a fast recovery kinetic mutant of dmBACCS2.