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Addgene

pT7-gRNA_zebrafish_mmp21
(Plasmid #72890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-gRNA
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zebrafish mmp21
  • Alt name
    martix metallopeptidase 21
  • gRNA/shRNA sequence
    CGGAGCTGATCACTGACA
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    ENSDART00000114949
  • Entrez Gene
    mmp21 (a.k.a. MMP-21)
  • Promoter T7 RNA pol

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer M13 Forward
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-gRNA_zebrafish_mmp21 was a gift from Nicholas Katsanis (Addgene plasmid # 72890 ; http://n2t.net/addgene:72890 ; RRID:Addgene_72890)

  • For your References section:

    A human laterality disorder caused by a homozygous deleterious mutation in MMP21. Perles Z, Moon S, Ta-Shma A, Yaacov B, Francescatto L, Edvardson S, Rein AJ, Elpeleg O, Katsanis N. J Med Genet. 2015 Dec;52(12):840-7. doi: 10.1136/jmedgenet-2015-103336. Epub 2015 Oct 1. 10.1136/jmedgenet-2015-103336 PubMed 26429889
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