pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
(Plasmid #72888)

• Purpose: fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
• Depositing Lab: Ute Hochgeschwender
• Publication: Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371/journal.pone.0059759. Epub 2013 Mar 27.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5427
• Total vector size (bp): 7759
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Gaussia luciferase
• Alt name: GLuc
• Species: Gaussia princeps
• Insert Size (bp): 555
• Promoter: CAG

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AAGCTTGCCACCATGGGAGTCAAAGTT
• 3′ sequencing primer: GGATCCCCGTCACCACCGGCCCCCTTGAT

Gene/Insert 2

• Gene/Insert name: Channelrhodopsin-2
• Alt name: ChR2
• Species: chlamydomonas
• Insert Size (bp): 930

Gene/Insert 3

• Gene/Insert name: enhanced yellow fluorescent protein
• Alt name: EYFP
• Species: synthetic
• Insert Size (bp): 720

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry