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Addgene

pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)
(Plasmid #72888)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72888 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 7759
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Gaussia luciferase
  • Alt name
    GLuc
  • Species
    Gaussia princeps
  • Insert Size (bp)
    555
  • Promoter CAG

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer AAGCTTGCCACCATGGGAGTCAAAGTT
  • 3′ sequencing primer GGATCCCCGTCACCACCGGCCCCCTTGAT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Channelrhodopsin-2
  • Alt name
    ChR2
  • Species
    chlamydomonas
  • Insert Size (bp)
    930

Gene/Insert 3

  • Gene/Insert name
    enhanced yellow fluorescent protein
  • Alt name
    EYFP
  • Species
    synthetic
  • Insert Size (bp)
    720

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP) was a gift from Ute Hochgeschwender (Addgene plasmid # 72888 ; http://n2t.net/addgene:72888 ; RRID:Addgene_72888)
  • For your References section:

    Light-emitting channelrhodopsins for combined optogenetic and chemical-genetic control of neurons. Berglund K, Birkner E, Augustine GJ, Hochgeschwender U. PLoS One. 2013;8(3):e59759. doi: 10.1371/journal.pone.0059759. Epub 2013 Mar 27. PONE-D-12-38868 [pii] PubMed 23544095