PMXS-NDI1
(Plasmid #72876)

• Purpose: expresses NDI1 in mammalian cells
• Depositing Lab: David Sabatini
• Publication: Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi: 10.1038/nature13110. Epub 2014 Mar 16.

Backbone

• Vector backbone: pMXs-IRES-blasticidin
• Backbone size w/o insert (bp): 5639
• Total vector size (bp): 7181
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NDI1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1542
• GenBank ID: 854919 NM_001182483.1
• Entrez Gene: NDI1 (a.k.a. YML120C)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (unknown if destroyed)
• 3′ cloning site: Xho1 (unknown if destroyed)
• 5′ sequencing primer: pLXSN 5' CCCTTGAACCTCCTCGTTCGACC

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The retroviral NDI1 vector was generated by cloning into the EcoRI and XhoI sites of the pMXS-ires-blast vector a cDNA insert generated by PCR using the primers below, followed by standard cloning techniques.
Ndi1 EcoRI F: ATGAATTCCATCACATCATCGAATTAC
Ndi1 XhoI R: ATCTCGAGAAAAGGGCATGTTAATTTCATCTATAAT
It is a retroviral plasmid so it may be recommended to be propagated at 30oc but author typically grows it at 37oC.