PMXS-SLC1A3
(Plasmid #72873)

• Purpose: The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
• Depositing Lab: David Sabatini
• Publication: Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10.1016/j.cell.2015.07.016.

Backbone

• Vector backbone: pMXs-IRES-blasticidin
• Backbone size w/o insert (bp): 5639
• Total vector size (bp): 7268
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SLC1A3
• Alt name: EA6; EAAT1; GLAST; GLAST1
• Species: H. sapiens (human)
• Insert Size (bp): 1629
• GenBank ID: NM_004172.4
• Entrez Gene: SLC1A3 (a.k.a. EA6, EAAT1, GLAST, GLAST1)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCCGGATCTAGCTAGTTAATTAAGccaccATGACTAAAAGCAATGGAGAAGAGCCC

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

It is a retroviral plasmid so it may be recommended to be propagated at 30°C but author typically grows it at 37°C.