pSK23
(Plasmid
#72851)
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Purposetemplate for C-terminal eGFP tagging, Puromycin-resistance
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72851 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJet1.2
- Backbone size w/o insert (bp) 2974
- Total vector size (bp) 4695
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Modifications to backbonealso contains a copia-Promoter + Puromycin resistance cassette, flanked by FRT sites
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Vector typeInsect Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP
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SpeciesAequorea victoria
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Insert Size (bp)800
- Promoter copia
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (unknown if destroyed)
- 3′ cloning site SpeI (unknown if destroyed)
- 5′ sequencing primer pJet fwd
- 3′ sequencing primer pJet rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bypEGFP-N1
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSK23 was a gift from Klaus Foerstemann (Addgene plasmid # 72851 ; http://n2t.net/addgene:72851 ; RRID:Addgene_72851) -
For your References section:
A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells. Kunzelmann S, Bottcher R, Schmidts I, Forstemann K. G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241. 10.1534/g3.116.028241 PubMed 27172193