pMHG-W57* reporter
(Plasmid #72850)

• Purpose: GFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.
• Depositing Lab: Daniel Liebler
• Publication: Halvey et al FEBS Open Bio. 2012 Dec;2:56-59. Epub 2012 Apr 17.

Backbone

• Vector backbone: pMONOhygro-gfp (pMHG)
• Backbone manufacturer: Invivogen
• Backbone size w/o insert (bp): 4728
• Modifications to backbone: Site-directed mutagenesis of GFP tryptophan 57 codon to a stop codon (W57⁄) (G171A, mRNA). An opal stop codon (TGA) was introduced using the following primers (forward, G CTG CCA GTG CCT TGA CCA ACT CTG GTG AC; reverse, GTC ACC AGA GTT GGT CAA AGG CAC TGG CAG C).
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP
• Species: H. sapiens (human)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry