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Addgene

PatoL-OG241
(Plasmid #72841)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72841 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    OG241
  • Backbone manufacturer
    Oxford Genetics
  • Total vector size (bp) 4695
  • Modifications to backbone
    Inserted PatoL promoter, atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM), upstream daGFP.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PatoL
  • Species
    E. coli DH5A
  • Insert Size (bp)
    552
  • Promoter PatoL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer TAAGCAACTAGTGCCGTGCATTGATGTATAAACT
  • 3′ sequencing primer TGCTTAGGTACCATCTAGTATTTCTCCTCTTTAATCTGCATACACCGTTGTGGGTACAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Capillary sequence files used the primers CATCTTCGATGGATAGCGATT and TGTACGGGATCTCCTTCTCG to verify MCS region including the promoter and the start codon of daGFP.

Please visit https://www.biorxiv.org/content/early/2016/01/07/035972 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PatoL-OG241 was a gift from Chris Barnes (Addgene plasmid # 72841 ; http://n2t.net/addgene:72841 ; RRID:Addgene_72841)

  • For your References section:

    Engineered acetoacetate-inducible whole-cell biosensors based on the AtoSC two-component system. Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, Donovan E, Ozdemir T, Thomas GM, Barnes CP. Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 10.1002/bit.27897. Epub 2021 Aug 9. 10.1002/bit.27897 PubMed 34289076
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