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AAVS1 T2 CRIPR in pX330 Citations (26)

Originally described in: Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors.
Natsume T, Kiyomitsu T, Saga Y, Kanemaki MT Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24.
PubMed Journal

Articles Citing AAVS1 T2 CRIPR in pX330

Articles
Retinoic acid and BMP4 cooperate with p63 to alter chromatin dynamics during surface epithelial commitment. Pattison JM, Melo SP, Piekos SN, Torkelson JL, Bashkirova E, Mumbach MR, Rajasingh C, Zhen HH, Li L, Liaw E, Alber D, Rubin AJ, Shankar G, Bao X, Chang HY, Khavari PA, Oro AE. Nat Genet. 2018 Dec;50(12):1658-1665. doi: 10.1038/s41588-018-0263-0. Epub 2018 Nov 5. PubMed
Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1. Bass TE, Cortez D. J Cell Biol. 2019 Apr 1;218(4):1235-1249. doi: 10.1083/jcb.201810058. Epub 2019 Feb 12. PubMed
Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies. Mair B, Tomic J, Masud SN, Tonge P, Weiss A, Usaj M, Tong AHY, Kwan JJ, Brown KR, Titus E, Atkins M, Chan KSK, Munsie L, Habsid A, Han H, Kennedy M, Cohen B, Keller G, Moffat J. Cell Rep. 2019 Apr 9;27(2):599-615.e12. doi: 10.1016/j.celrep.2019.02.041. PubMed

Associated Plasmids

Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole. Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT. Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010. PubMed

Associated Plasmids

Microhomology based CRISPR tagging tools for protein tracking, purification, and depletion. Lin DW, Chung BP, Huang JW, Wang X, Huang L, Kaiser P. J Biol Chem. 2019 May 28. pii: RA119.008422. doi: 10.1074/jbc.RA119.008422. PubMed

Associated Plasmids

A unified allosteric/torpedo mechanism for transcriptional termination on human protein-coding genes. Eaton JD, Francis L, Davidson L, West S. Genes Dev. 2020 Jan 1;34(1-2):132-145. doi: 10.1101/gad.332833.119. Epub 2019 Dec 5. PubMed
The role of Mediator and Little Elongation Complex in transcription termination. Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, Hirose H, Sasaki K, Abe R, Chen K, He Y, Zhang Y, Takigawa I, Tsukiyama T, Watanabe M, Fujii S, Iida M, Yamamoto J, Yamaguchi Y, Suzuki Y, Matsumoto M, Nakayama KI, Washburn MP, Saraf A, Florens L, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Hatakeyama S. Nat Commun. 2020 Feb 26;11(1):1063. doi: 10.1038/s41467-020-14849-1. PubMed
CTCF is dispensable for immune cell transdifferentiation but facilitates an acute inflammatory response. Stik G, Vidal E, Barrero M, Cuartero S, Vila-Casadesus M, Mendieta-Esteban J, Tian TV, Choi J, Berenguer C, Abad A, Borsari B, le Dily F, Cramer P, Marti-Renom MA, Stadhouders R, Graf T. Nat Genet. 2020 Jul;52(7):655-661. doi: 10.1038/s41588-020-0643-0. Epub 2020 Jun 8. PubMed
Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication. Kumagai A, Dunphy WG. Cell Rep. 2020 Sep 22;32(12):108178. doi: 10.1016/j.celrep.2020.108178. PubMed
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Sapoznik E, Chang BJ, Huh J, Ju RJ, Azarova EV, Pohlkamp T, Welf ES, Broadbent D, Carisey AF, Stehbens SJ, Lee KM, Marin A, Hanker AB, Schmidt JC, Arteaga CL, Yang B, Kobayashi Y, Tata PR, Kruithoff R, Doubrovinski K, Shepherd DP, Millett-Sikking A, York AG, Dean KM, Fiolka RP. Elife. 2020 Nov 12;9. pii: 57681. doi: 10.7554/eLife.57681. PubMed
Chromosomal localization of Ewing sarcoma EWSR1/FLI1 protein promotes the induction of aneuploidy. Park H, Kim H, Hassebroek V, Azuma Y, Slawson C, Azuma M. J Biol Chem. 2021 Jan-Jun;296:100164. doi: 10.1074/jbc.RA120.014328. Epub 2020 Dec 10. PubMed
SLX4-XPF mediates DNA damage responses to replication stress induced by DNA-protein interactions. Ishimoto R, Tsuzuki Y, Matsumura T, Kurashige S, Enokitani K, Narimatsu K, Higa M, Sugimoto N, Yoshida K, Fujita M. J Cell Biol. 2021 Jan 4;220(1). pii: 211628. doi: 10.1083/jcb.202003148. PubMed
Bora phosphorylation substitutes in trans for T-loop phosphorylation in Aurora A to promote mitotic entry. Tavernier N, Thomas Y, Vigneron S, Maisonneuve P, Orlicky S, Mader P, Regmi SG, Van Hove L, Levinson NM, Gasmi-Seabrook G, Joly N, Poteau M, Velez-Aguilera G, Gavet O, Castro A, Dasso M, Lorca T, Sicheri F, Pintard L. Nat Commun. 2021 Mar 26;12(1):1899. doi: 10.1038/s41467-021-21922-w. PubMed
Pluripotent stem cell model of early hematopoiesis in Down syndrome reveals quantitative effects of short-form GATA1 protein on lineage specification. Matsuo S, Nishinaka-Arai Y, Kazuki Y, Oshimura M, Nakahata T, Niwa A, Saito MK. PLoS One. 2021 Mar 29;16(3):e0247595. doi: 10.1371/journal.pone.0247595. eCollection 2021. PubMed
Measuring nonhomologous end-joining, homologous recombination and alternative end-joining simultaneously at an endogenous locus in any transfectable human cell. Hussain SS, Majumdar R, Moore GM, Narang H, Buechelmaier ES, Bazil MJ, Ravindran PT, Leeman JE, Li Y, Jalan M, Anderson KS, Farina A, Soni R, Mohibullah N, Hamzic E, Rong-Mullins X, Sifuentes C, Damerla RR, Viale A, Powell SN, Higginson DS. Nucleic Acids Res. 2021 Jul 21;49(13):e74. doi: 10.1093/nar/gkab262. PubMed
A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9. Mair B, Aregger M, Tong AHY, Chan KSK, Moffat J. Methods Mol Biol. 2022;2377:1-27. doi: 10.1007/978-1-0716-1720-5_1. PubMed
HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51. Anand R, Buechelmaier E, Belan O, Newton M, Vancevska A, Kaczmarczyk A, Takaki T, Rueda DS, Powell SN, Boulton SJ. Nature. 2022 Jan;601(7892):268-273. doi: 10.1038/s41586-021-04261-0. Epub 2021 Dec 22. PubMed
Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes. Hindul NL, Jhita A, Oprea DG, Hussain TA, Gonchar O, Campillo MAM, O'Regan L, Kanemaki MT, Fry AM, Hirota K, Tanaka K. Biol Open. 2022 Feb 15;11(2). pii: 274087. doi: 10.1242/bio.059056. Epub 2022 Feb 16. PubMed

Associated Plasmids

Mammalian monocarboxylate transporter 7 (MCT7/Slc16a6) is a novel facilitative taurine transporter. Higuchi K, Sugiyama K, Tomabechi R, Kishimoto H, Inoue K. J Biol Chem. 2022 Apr;298(4):101800. doi: 10.1016/j.jbc.2022.101800. Epub 2022 Mar 5. PubMed
Establishment of a luciferase-based method for measuring cancer cell adhesion and proliferation. Jessen SB, Ozkul DC, Ozen Y, Gogenur I, Troelsen JT. Anal Biochem. 2022 Aug 1;650:114723. doi: 10.1016/j.ab.2022.114723. Epub 2022 May 12. PubMed
The 3' Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies' association with histone locus bodies. Suzuki H, Abe R, Shimada M, Hirose T, Hirose H, Noguchi K, Ike Y, Yasui N, Furugori K, Yamaguchi Y, Toyoda A, Suzuki Y, Yamamoto T, Saitoh N, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Takahashi H. Nat Commun. 2022 May 25;13(1):2905. doi: 10.1038/s41467-022-30632-w. PubMed
Human papillomavirus associated XPF deficiency increases alternative end joining and cisplatin sensitivity in head and neck squamous cell carcinoma. Zuo N, Ma L, Liu T, Hu W, Luo Y, Meng H, Ren Q, Deng Y, Wei L, Liu Q. Oral Oncol. 2023 May;140:106367. doi: 10.1016/j.oraloncology.2023.106367. Epub 2023 Mar 28. PubMed
Monocarboxylate Transporter 13 (MCT13/SLC16A13) Functions as a Novel Plasma Membrane Oligopeptide Transporter. Higuchi K, Kunieda M, Sugiyama K, Tomabechi R, Kishimoto H, Inoue K. Nutrients. 2023 Aug 10;15(16):3527. doi: 10.3390/nu15163527. PubMed
DOT1L stimulates MYC/Mondo transcription factor activity by promoting its degradation cycle on chromatin. Sepulveda GP, Gushchanskaia ES, Mora-Martin A, Esse R, Nikorich I, Ceballos A, Kwan J, Blum BC, Dholiya P, Emili A, Perissi V, Cardamone MD, Grishok A. bioRxiv [Preprint]. 2024 Feb 7:2024.02.06.579191. doi: 10.1101/2024.02.06.579191. PubMed
Longitudinal Magnetic Resonance Imaging Tracking of Transplanted Neural Progenitor Cells in the Spinal Cord Utilizing the Bright-Ferritin Mechanism. Luo Z, Zhuang K, Kim SJ, Vollett KDW, Lou Z, Wang J, Cheng HM, Khazaei M, Fehlings MG, Cheng HM. Stem Cells Transl Med. 2024 Jun 14;13(6):546-558. doi: 10.1093/stcltm/szae016. PubMed
Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism. Pujadas Liwag EM, Wei X, Acosta N, Carter LM, Yang J, Almassalha LM, Jain S, Daneshkhah A, Rao SSP, Seker-Polat F, MacQuarrie KL, Ibarra J, Agrawal V, Aiden EL, Kanemaki MT, Backman V, Adli M. Genome Biol. 2024 Mar 22;25(1):77. doi: 10.1186/s13059-024-03212-y. PubMed

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