pMK277 (mClover-NeoR)
(Plasmid #72793)

• Purpose: C-terminal tagging with mClover using CRIPSR/Cas9
• Depositing Lab: Masato Kanemaki
• Publication: Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 10.1016/j.celrep.2016.03.001. Epub 2016 Mar 24.

Backbone

• Vector backbone: pBluescript KS(-)
• Backbone size w/o insert (bp): 3038
• Total vector size (bp): 5924
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mClover-Neo
• Species: H. sapiens (human), M. musculus (mouse), R. norvegicus (rat), G. gallus (chicken)
• Insert Size (bp): 2886
• Tag / Fusion Protein:
  • mClover (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: GGAAACAGCTATGACCATG

Resource Information

• Supplemental Documents:
  • pMK277 (mClover-NeoR)
• A portion of this plasmid was derived from a plasmid made by: mClover is a kind gift from Dr. Michael Lin (Addgene #40259)(Lam et al. Nat. Meth., 2012).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector can be used for the construction of donor vectors for tagging with mClover.