pRFP shRNA-resistant TrkB F616A -S478A
(Plasmid
#72718)
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PurposeMammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTrkB
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SpeciesR. norvegicus (rat)
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MutationshRNA-resistant TrkB (T300C, T301A, C302G, C306A, T307C) and F616A -S478A
- Promoter CMV
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Tag
/ Fusion Protein
- RFP (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer DsRed1-N (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRFP shRNA-resistant TrkB F616A -S478A was a gift from Freddy Jeanneteau (Addgene plasmid # 72718 ; http://n2t.net/addgene:72718 ; RRID:Addgene_72718) -
For your References section:
Neurotrophic-priming of glucocorticoid receptor signaling is essential for neuronal plasticity to stress and antidepressant treatment. Arango-Lievano M, Lambert WM, Bath KG, Garabedian MJ, Chao MV, Jeanneteau F. Proc Natl Acad Sci U S A. 2015 Nov 16. pii: 201509045. 10.1073/pnas.1509045112 PubMed 26630005