pGL4.10 Dub3
(Plasmid #72684)

• Purpose: luciferase reporter with Dub3 promoter
• Depositing Lab: Domenico Maiorano
• Publication: van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 10.1371/journal.pone.0093663. eCollection 2014.

Backbone

• Vector backbone: pGL4.10[luc]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4242
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dub3 promoter
• Alt name: ubiquitin specific peptidase 17-like E
• Species: M. musculus (mouse)
• Insert Size (bp): 3200
• Entrez Gene: Usp17le (a.k.a. DUB-3, Dub3, Dub6, EG625530, Gm6596, USP17, USP17-E, Usp17l2)
• Promoter: Dub3
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS found a small number of single nucleotide discrepancies relative to Sanger sequencing results provided by the depositing laboratory. These do not affect plasmid function