pcDNA3-Dub3 mouse
(Plasmid
#72682)
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Purposemammalian expression of mouse Dub3
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3-HA
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDub3
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Alt nameubiquitin specific peptidase 17-like E
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SpeciesM. musculus (mouse)
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Entrez GeneUsp17le (a.k.a. DUB-3, Dub3, Dub6, EG625530, Gm6596, USP17, USP17-E, Usp17l2)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene Sanger sequencing detected an S4A mutation within the Dub3 translation that should not affect protein function
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Dub3 mouse was a gift from Domenico Maiorano (Addgene plasmid # 72682 ; http://n2t.net/addgene:72682 ; RRID:Addgene_72682) -
For your References section:
High Dub3 expression in mouse ESCs couples the G1/S checkpoint to pluripotency. van_der_Laan S, Tsanov N, Crozet C, Maiorano D. Mol Cell. 2013 Nov 7;52(3):366-79. doi: 10.1016/j.molcel.2013.10.003. 10.1016/j.molcel.2013.10.003 PubMed 24207026