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Addgene

pCas1+2
(Plasmid #72676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-1b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Streptomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas1, Cas2
  • Species
    from K12 genome
  • Insert Size (bp)
    1204

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas1+2 was a gift from Udi Qimron (Addgene plasmid # 72676 ; http://n2t.net/addgene:72676 ; RRID:Addgene_72676)

  • For your References section:

    Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli. Yosef I, Goren MG, Qimron U. Nucleic Acids Res. 2012 Jul;40(12):5569-76. doi: 10.1093/nar/gks216. Epub 2012 Mar 8. 10.1093/nar/gks216 PubMed 22402487
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