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Addgene

pKPY737
(Plasmid #72673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2544
  • Total vector size (bp) 5358
  • Vector type
    Gateway Entry Clone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    2814
  • Mutation
    Thr412Gly in CePheRS Alpha Subunit

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKPY737 was a gift from David Tirrell (Addgene plasmid # 72673 ; http://n2t.net/addgene:72673 ; RRID:Addgene_72673)
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