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Addgene

pKPY115
(Plasmid #72669)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72669 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD33
  • Backbone manufacturer
    Beckwith Lab
  • Backbone size w/o insert (bp) 5319
  • Total vector size (bp) 8717
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thr251Gly-EcPheRS
  • Alt name
    E. coli Phenylalanyl-tRNA Synthetase (Thr251Gly in Alpha Subunit)
  • Species
    E. coli
  • Insert Size (bp)
    3398
  • Mutation
    Thr251Gly in Alpha Subunit
  • Promoter PBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SphI (not destroyed)
  • 5′ sequencing primer pBAD Forward
  • 3′ sequencing primer pBAD Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKPY115 was a gift from David Tirrell (Addgene plasmid # 72669 ; http://n2t.net/addgene:72669 ; RRID:Addgene_72669)
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