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Addgene

pLicC-MBP-APETx2
(Plasmid #72668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLicC-MBP
  • Backbone manufacturer
    Geneart (Invitrogen)
  • Total vector size (bp) 6554
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APETx2
  • Species
    Sea anemone
  • Insert Size (bp)
    200
  • Promoter T7
  • Tags / Fusion Proteins
    • MalE signal sequence for periplasmic export (N terminal on backbone)
    • His tag (N terminal on backbone)
    • MBP (N terminal on backbone)
    • TEV site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV/KpnI (not destroyed)
  • 3′ cloning site SacI/SalI/HindIII/NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Purchased from Genart
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLicC-MBP-APETx2 was a gift from Glenn King (Addgene plasmid # 72668 ; http://n2t.net/addgene:72668 ; RRID:Addgene_72668)

  • For your References section:

    Functional expression in Escherichia coli of the disulfide-rich sea anemone peptide APETx2, a potent blocker of acid-sensing ion channel 3. Anangi R, Rash LD, Mobli M, King GF. Mar Drugs. 2012 Jul;10(7):1605-18. doi: 10.3390/md10071605. Epub 2012 Jul 23. 10.3390/md10071605 PubMed 22851929
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