H2B-GFP-AsLOV2-NES27 (pDN158)
(Plasmid
#72659)
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PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72659 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5585
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH2B-GFP-AsLOV2-NES27
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
H2B-GFP-AsLOV2-NES27 (pDN158) was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 72659 ; http://n2t.net/addgene:72659 ; RRID:Addgene_72659) -
For your References section:
Optogenetic control of nuclear protein export. Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B. Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624. 10.1038/ncomms10624 PubMed 26853913
