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Addgene

pWPXL-NP1
(Plasmid #72654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWPXL
  • Backbone manufacturer
    Trono lab (Addgene plasmid 12257)
  • Backbone size w/o insert (bp) 10510
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NP1
  • Alt name
    Neuronal Pentraxin 1
  • Alt name
    NPTX1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1302
  • GenBank ID
    NM_153735.2
  • Entrez Gene
    Nptx1
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EF1a-Fwd
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPXL-NP1 was a gift from Ramon Trullas (Addgene plasmid # 72654 ; http://n2t.net/addgene:72654 ; RRID:Addgene_72654)

  • For your References section:

    Neuronal pentraxin 1 negatively regulates excitatory synapse density and synaptic plasticity. Figueiro-Silva J, Gruart A, Clayton KB, Podlesniy P, Abad MA, Gasull X, Delgado-Garcia JM, Trullas R. J Neurosci. 2015 Apr 8;35(14):5504-21. doi: 10.1523/JNEUROSCI.2548-14.2015. 10.1523/JNEUROSCI.2548-14.2015 PubMed 25855168
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