pJB128 (MatP-mEos2)
(Plasmid #72653)

• Purpose: Inducible expression of MatP-mEos2 in bacteria
• Depositing Lab: Jie Xiao
• Publication: Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. doi: 10.1371/journal.pgen.1005128. eCollection 2015 Apr.

Backbone

• Vector backbone: pDR122
• Backbone manufacturer: P. de Boer (Hale et al. EMBO J 2001)
• Backbone size w/o insert (bp): 7839
• Total vector size (bp): 9005
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For fluorescence studies, grow at RT or 30C.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MatP-mEos2
• Species: E. coli
• Insert Size (bp): 1166
• Promoter: T5-lac
• Tag / Fusion Protein:
  • mEos2 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SnaBI (not destroyed)
• 5′ sequencing primer: taatacgactcactatagg
• 3′ sequencing primer: GGG CCT CTT CGC TAT T

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry