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Addgene

pDEST1K18ms
(Plasmid #72646)

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Item Catalog # Description Quantity Price (USD)
Plasmid 72646 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pDEST1K18ms
  • Backbone size (bp) 7450
  • Modifications to backbone
    LR clonase Gateway compatible
  • Vector type
    sucrose counter-selection allelic exchange vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    The strain grows to a lower final turbidity than other E coli strains and is cold sensitive. Do not store @ 4C.
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TGTAAAACGACGGCCAGT
  • 3′ sequencing primer AACAGCTATGACATGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST1K18ms was a gift from Brian Kvitko (Addgene plasmid # 72646 ; http://n2t.net/addgene:72646 ; RRID:Addgene_72646)

  • For your References section:

    Patterns of Seed-to-Seedling Transmission of Xanthomonas citri pv. malvacearum, the Causal Agent of Cotton Bacterial Blight. Mijatovic J, Severns PM, Kemerait RC, Walcott RR, Kvitko BH. Phytopathology. 2021 Dec;111(12):2176-2184. doi: 10.1094/PHYTO-02-21-0057-R. Epub 2021 Nov 22. 10.1094/PHYTO-02-21-0057-R PubMed 34032522
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