zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
(Plasmid #72638)

• Purpose: Tol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domain
• Depositing Lab: Joshua Bonkowsky
• Publication: Bonkowsky lab FingR plasmids (unpublished)

Backbone

• Vector backbone: pDONR 221
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6945
• Vector type: Gateway

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
• Species: M. musculus (mouse)
• Insert Size (bp): 2478

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: from Don Arnold lab (Gross et al., Neuron, 2013): Plasmids pCAG_PSD95.FingR-eGFP-CCR5TC-KRAB(A) and pCAG_GPHN.FingR-mKate2-IL2RGTCKRAB(A) were obtained from Addgene (plasmids #46295 and #46297).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Details of cloning are in:
Son et al., Scientific Reports, 2016. Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons.