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Addgene

pSG5-BMLF1
(Plasmid #72636)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4184
  • Total vector size (bp) 5684
  • Modifications to backbone
    polylinker EcoRI, Sac1, Kpn1, Sma1, Xma1, BamH1, Xba1, Sal1, Acc1, HincII, Pst1, Sph1, HindIII, BglII
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BMLF1/BSLF2
  • Alt name
    Mta
  • Species
    virus
  • Insert Size (bp)
    1500
  • Mutation
    Q157H
  • GenBank ID
    V01555.2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-BMLF1 was a gift from S. Diane Hayward (Addgene plasmid # 72636 ; http://n2t.net/addgene:72636 ; RRID:Addgene_72636)

  • For your References section:

    A replication function associated with the activation domain of the Epstein-Barr virus Zta transactivator. Sarisky RT, Gao Z, Lieberman PM, Fixman ED, Hayward GS, Hayward SD. J Virol. 1996 Dec;70(12):8340-7. PubMed 8970953
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