pSG5-BMRF1
(Plasmid
#72631)
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PurposeMammalian expression vector for Epstein-Barr virus polymerase accessory protein
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4184
- Total vector size (bp) 5384
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Modifications to backbonepolylinker EcoRI, Sac1, Kpn1, Sma1, Xma1, BamH1, Xba1, Sal1, Acc1, HincII, Pst1, Sph1, HindIII, BglII
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBMRF1
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Alt namepolymerase accessory protein
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Speciesvirus
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Insert Size (bp)1200
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GenBank IDV01555.2
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Tag
/ Fusion Protein
- D22G and S349N
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xba1 (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-BMRF1 was a gift from S. Diane Hayward (Addgene plasmid # 72631 ; http://n2t.net/addgene:72631 ; RRID:Addgene_72631) -
For your References section:
A replication function associated with the activation domain of the Epstein-Barr virus Zta transactivator. Sarisky RT, Gao Z, Lieberman PM, Fixman ED, Hayward GS, Hayward SD. J Virol. 1996 Dec;70(12):8340-7. PubMed 8970953
Map uploaded by the depositor.
