pSLIK 3XFLAG-wtGATA6-3XAU1 neo
(Plasmid #72618)

• Purpose: Expresses an inducible, N-terminally 3X-FLAG tagged and C-terminally 3X-AU1 tagged wtGATA6
• Depositing Lab: Kevin Janes
• Publication: Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.

Backbone

• Vector backbone: PSLIKneo
• Backbone manufacturer: Addgene #25735
• Backbone size w/o insert (bp): 13286
• Total vector size (bp): 14029
• Modifications to backbone: Followed standard LR recombination protocols
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GATA binding protein 6
• Alt name: GATA6
• Species: H. sapiens (human)
• Insert Size (bp): 1917
• GenBank ID: NM_005257
• Entrez Gene: GATA6
• Promoter: TRE Tight
• Tags / Fusion Proteins:
  • 3XFLAG (N terminal on insert)
  • 3XAU1 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hUBCpro-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry