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pBabe 3XFLAG-T34A-GATA6-3XAU1 puro
(Plasmid #72609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABEpuro
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 7817
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA binding protein 6
  • Alt name
    GATA6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1917
  • Mutation
    changed threonine34 to alanine
  • GenBank ID
    NM_005257
  • Entrez Gene
    GATA6
  • Tags / Fusion Proteins
    • 3XFLAG (N terminal on insert)
    • 3XAU1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer pBABE3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe 3XFLAG-T34A-GATA6-3XAU1 puro was a gift from Kevin Janes (Addgene plasmid # 72609 ; http://n2t.net/addgene:72609 ; RRID:Addgene_72609)

  • For your References section:

    TNF-insulin crosstalk at the transcription factor GATA6 is revealed by a model that links signaling and transcriptomic data tensors. Chitforoushzadeh Z, Ye Z, Sheng Z, LaRue S, Fry RC, Lauffenburger DA, Janes KA. Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373. 10.1126/scisignal.aad3373 PubMed 27273097
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