pBabe 3XFLAG-S37A-GATA6-3XAU1 puro
(Plasmid #72608)

• Purpose: For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with a single serine#37 to alanine mutation
• Depositing Lab: Kevin Janes
• Publication: Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.

Backbone

• Vector backbone: pBABEpuro
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 7817
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GATA binding protein 6
• Alt name: GATA6
• Species: H. sapiens (human)
• Insert Size (bp): 1917
• Mutation: changed serine37 to alanine
• GenBank ID: NM_005257
• Entrez Gene: GATA6
• Tags / Fusion Proteins:
  • 3XFLAG (N terminal on insert)
  • 3XAU1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE5'
• 3′ sequencing primer: pBABE3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry