Skip to main content
Addgene

pEGFP-2A-TAL6-MSTN
(Plasmid #72597)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72597 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pTAL6
  • Backbone manufacturer
    Han lab
  • Vector type
    Mammalian Expression, TALEN
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TALEN for MSTN
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To streamline the assembly of a functional pair of TALENs into one plasmid, we placed two GoldyTALEN scaffolds linked by a self-cleaving 2A peptide into a final plasmid designated as pTAL6, which has a unique BsaI site instead of BsmBI site within the second GoldyTALEN scaffold.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-2A-TAL6-MSTN was a gift from Renzhi Han (Addgene plasmid # 72597 ; http://n2t.net/addgene:72597 ; RRID:Addgene_72597)

  • For your References section:

    Targeted Myostatin Gene Editing in Multiple Mammalian Species Directed by a Single Pair of TALE Nucleases. Xu L, Zhao P, Mariano A, Han R. Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 10.1038/mtna.2013.39. 10.1038/mtna.2013.39 PubMed 23900226
Related items:
Commonly requested with: