pRK5-FLAG-Sestrin1 isoform1
(Plasmid #72594)

• Purpose: overexpression, codon optimized for mammalian expression
• Depositing Lab: David Sabatini
• Publication: Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. Epub 2014 Sep 25.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4661
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SESN1
• Alt name: PA26; SEST1
• Species: H. sapiens (human)
• Insert Size (bp): 1656
• Mutation: codon optimized for mammalian expression, see deposited sequence
• GenBank ID: NM_014454.2
• Entrez Gene: SESN1 (a.k.a. PA26, SEST1)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: SP6 : ATT TAG GTG ACA CTA TAG
• 3′ sequencing primer: pRK5-3': TGT AAC CAT TAT AAG CTG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry