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hGDF8 donor CFP-hDF
(Plasmid #72591)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72591 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAVS1 SA-2A-puro-pA donor
  • Backbone manufacturer
    Rudolf Jaenisch (Addgene plasmid # 22075)
  • Backbone size w/o insert (bp) 6602
  • Total vector size (bp) 14889
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dysferlin
  • Species
    H. sapiens (human)
  • Entrez Gene
    DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
  • Promoter CMV
  • Tag / Fusion Protein
    • ECFP (N terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette. ccdB gene is nonfunctional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hGDF8 donor CFP-hDF was a gift from Renzhi Han (Addgene plasmid # 72591 ; http://n2t.net/addgene:72591 ; RRID:Addgene_72591)

  • For your References section:

    Targeted Myostatin Gene Editing in Multiple Mammalian Species Directed by a Single Pair of TALE Nucleases. Xu L, Zhao P, Mariano A, Han R. Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 10.1038/mtna.2013.39. 10.1038/mtna.2013.39 PubMed 23900226
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