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pLP-mEos2-Cep123
(Plasmid #72589)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72589 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLP-mEos2
  • Backbone manufacturer
    Modified Clontech vector
  • Backbone size w/o insert (bp) 5781
  • Total vector size (bp) 8133
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cep123
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2352
  • GenBank ID
    NM_032816
  • Entrez Gene
    CEP89 (a.k.a. CCDC123, CEP123)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal I (not destroyed)
  • 3′ cloning site BamH I (not destroyed)
  • 5′ sequencing primer mEos2 seq2
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLP-mEos2-Cep123 was a gift from Michel Bornens (Addgene plasmid # 72589 ; http://n2t.net/addgene:72589 ; RRID:Addgene_72589)

  • For your References section:

    Assessing the localization of centrosomal proteins by PALM/STORM nanoscopy. Sillibourne JE, Specht CG, Izeddin I, Hurbain I, Tran P, Triller A, Darzacq X, Dahan M, Bornens M. Cytoskeleton (Hoboken). 2011 Nov;68(11):619-27. doi: 10.1002/cm.20536. Epub 2011 Oct 18. 10.1002/cm.20536 PubMed 21976302
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