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pHis6-hDF
(Plasmid #72585)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72585 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 11000
  • Modifications to backbone
    EGFP replaced with His
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dysferlin
  • Species
    H. sapiens (human)
  • Entrez Gene
    DYSF (a.k.a. FER1L1, LGMD2B, LGMDR2, MMD1)
  • Promoter CMV
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis6-hDF was a gift from Renzhi Han (Addgene plasmid # 72585 ; http://n2t.net/addgene:72585 ; RRID:Addgene_72585)

  • For your References section:

    Dysferlin forms a dimer mediated by the C2 domains and the transmembrane domain in vitro and in living cells. Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R. PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub 2011 Nov 14. 10.1371/journal.pone.0027884 PubMed 22110769
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