pRK5-myc-PICK1-rescue
(Plasmid #72573)

• Purpose: Expresses myc-PICK1 shRNA resistant cDNA
• Depositing Labs: Victor Anggono, Richard Huganir
• Publication: Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. doi: 10.1523/JNEUROSCI.5633-10.2011.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 5307
• Total vector size (bp): 6555
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PICK1
• Species: R. norvegicus (rat)
• Insert Size (bp): 1248
• Mutation: 5 silent mutations around shRNA recognition sequence
• GenBank ID: NM_053460.1
• Entrez Gene: Pick1 (a.k.a. Prkcabp)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ggtgacactatagaataacatccac
• 3′ sequencing primer: cccgatcgatccagacatgataag

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry