MSCV-NSD3-short
(Plasmid
#72552)
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Purposeexpresses 3*FLAG tagged human NSD3-short
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV-PIG
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Backbone manufacturerAddgene Plasmid #18751
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 6500
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Modifications to backbonenone
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNSD3-short
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SpeciesH. sapiens (human)
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Insert Size (bp)1937
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Mutationnone
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GenBank IDNM_017778.2
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Entrez GeneNSD3 (a.k.a. KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328)
- Promoter LTR
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Tag
/ Fusion Protein
- 3*FLAG (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MSCV-NSD3-short was a gift from Christopher Vakoc (Addgene plasmid # 72552 ; http://n2t.net/addgene:72552 ; RRID:Addgene_72552) -
For your References section:
NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 Chromatin Remodeler. Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, Pappin DJ, Joshua-Tor L, Vakoc CR. Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 10.1016/j.molcel.2015.10.033. 10.1016/j.molcel.2015.10.033 PubMed 26626481