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Addgene

MSCV-NSD3-short W284A
(Plasmid #72551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-PIG
  • Backbone manufacturer
    Addgene Plasmid #18751
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 6500
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NSD3-short
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1937
  • Mutation
    W284A
  • GenBank ID
    NM_017778.2
  • Entrez Gene
    NSD3 (a.k.a. KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3*FLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-NSD3-short W284A was a gift from Christopher Vakoc (Addgene plasmid # 72551 ; http://n2t.net/addgene:72551 ; RRID:Addgene_72551)

  • For your References section:

    NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 Chromatin Remodeler. Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, Pappin DJ, Joshua-Tor L, Vakoc CR. Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 10.1016/j.molcel.2015.10.033. 10.1016/j.molcel.2015.10.033 PubMed 26626481
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