pcDNA-ET-I654Q
(Plasmid
#72545)
-
Purposeexpresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 72545 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA 3
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 5700
-
Modifications to backbonenone
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBRD4 ET
-
SpeciesH. sapiens (human)
-
Insert Size (bp)270
-
MutationI654Q
-
GenBank IDNM_058243.2
-
Entrez GeneBRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
-
Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-ET-I654Q was a gift from Christopher Vakoc (Addgene plasmid # 72545 ; http://n2t.net/addgene:72545 ; RRID:Addgene_72545) -
For your References section:
NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 Chromatin Remodeler. Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, Pappin DJ, Joshua-Tor L, Vakoc CR. Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 10.1016/j.molcel.2015.10.033. 10.1016/j.molcel.2015.10.033 PubMed 26626481