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Addgene

pJK618
(Plasmid #72535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 8435
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, small subunit
  • Alt name
    purS
  • Alt name
    AZ09_06375
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene
  • GenBank ID
    KDE20458
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
  • Alt name
    purQ
  • Alt name
    AZ09_06385
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE20460
  • Promoter n/a

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, large subunit
  • Alt name
    purL
  • Alt name
    AZ09_06390
  • Species
    Acetobacter aceti 1023
  • GenBank ID
    KDE20461
  • Promoter n/a
  • Tag / Fusion Protein
    • His6 (C terminal on backbone)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Three overlapping genes from single operon

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK618 was a gift from T. J. Kappock (Addgene plasmid # 72535 ; http://n2t.net/addgene:72535 ; RRID:Addgene_72535)
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