pJK613
(Plasmid #72534)

• Purpose: Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
• Depositing Lab: T. J. Kappock
• Publication: A multisubunit Gram-negative FGAM synthetase (unpublished)

Backbone

• Vector backbone: pET23a
• Backbone size w/o insert (bp): 3666
• Total vector size (bp): 6938
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: formylglycinamidine ribonucleotide synthetase, small subunit
• Alt name: purS
• Alt name: AZ09_06375
• Species: Acetobacter aceti 1023
• Mutation: synthetic gene
• GenBank ID: KDE20458
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: hypothetical protein in purSQL operon
• Alt name: OrfY
• Alt name: AZ09_06380
• Species: Acetobacter aceti 1023
• Mutation: residues 1-69 only; C-terminal domain removed
• GenBank ID: KDE20459
• Promoter: n/a

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: formylglycinamidine ribonucleotide synthetase, glutaminase subunit
• Alt name: purQ
• Alt name: AZ09_06385
• Species: Acetobacter aceti 1023
• GenBank ID: KDE20460
• Promoter: n/a

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: formylglycinamidine ribonucleotide synthetase, large subunit
• Alt name: purL
• Alt name: AZ09_06390
• Species: Acetobacter aceti 1023
• GenBank ID: KDE20461
• Promoter: n/a
• Tag / Fusion Protein:
  • His6 (C terminal on backbone)

Cloning Information for Gene/Insert 4

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Four overlapping genes in one operon