pJK577
(Plasmid
#72528)
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PurposeEncodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 2710
- Total vector size (bp) 2703
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Modifications to backboneremoved NdeI (vector) - NdeI (insert) segment, deleting 150 bp of vector (including lacZ promoter and M13-forward primer binding site)
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Vector typeGene synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameformylglycinamidine ribonucleotide synthetase, small subunit
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Alt namepurS
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Alt nameAZ09_06375
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SpeciesAcetobacter aceti 1023
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GenBank IDKDE20458
- Promoter n/a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (destroyed during cloning)
- 3′ cloning site EcoRV (destroyed during cloning)
- 5′ sequencing primer none (AmpR)
- 3′ sequencing primer M13-reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK577 was a gift from T. J. Kappock (Addgene plasmid # 72528 ; http://n2t.net/addgene:72528 ; RRID:Addgene_72528)