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Addgene

pJK576
(Plasmid #72527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57
  • Backbone size w/o insert (bp) 2710
  • Total vector size (bp) 3071
  • Modifications to backbone
    removed NdeI (vector) - NdeI (insert) segment, deleting 150 bp of vector (including lacZ promoter and M13-forward primer binding site)
  • Vector type
    Gene synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    formylglycinamidine ribonucleotide synthetase, small subunit
  • Alt name
    purS
  • Alt name
    AZ09_06375
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene
  • GenBank ID
    KDE20458
  • Promoter n/a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer none (AmpR)
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    hypothetical protein in purSQL operon
  • Alt name
    OrfY
  • Alt name
    AZ09_06380
  • Species
    Acetobacter aceti 1023
  • Mutation
    synthetic gene
  • GenBank ID
    KDE20459
  • Promoter n/a

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The region 3' to the orfY gene includes partial sequence of purQ needed for cassette cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK576 was a gift from T. J. Kappock (Addgene plasmid # 72527 ; http://n2t.net/addgene:72527 ; RRID:Addgene_72527)
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