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Addgene

pJK546
(Plasmid #72518)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72518 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 5723
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hypothetical protein in purSQL operon
  • Alt name
    orfY
  • Alt name
    AZ09_06380
  • Species
    Acetobacter aceti 1023
  • Mutation
    contains additional Ala2
  • GenBank ID
    KDE20459
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pET upstream primer
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJK546 was a gift from T. J. Kappock (Addgene plasmid # 72518 ; http://n2t.net/addgene:72518 ; RRID:Addgene_72518)
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