CFP-GIV-F1685A
(Plasmid
#72489)
-
PurposeMammalian expression of The CFP-tagged GIV-F1685A biosensors
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 72489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 5848
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameGIV/Girdin
-
Alt nameGIV-F1685A
-
SpeciesH. sapiens (human)
-
Insert Size (bp)5610
-
MutationF1685A
-
GenBank IDBAE44387.1, AB201172.1
-
Entrez GeneCCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
- Promoter CMV
-
Tag
/ Fusion Protein
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGHRev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CFP-GIV-F1685A was a gift from Pradipta Ghosh (Addgene plasmid # 72489 ; http://n2t.net/addgene:72489 ; RRID:Addgene_72489)