pJK520
(Plasmid #72479)

• Purpose: Encodes Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); hypothetical protein with N-terminal His6 tag (H6AaOrfY), synthetic gene
• Depositing Lab: T. J. Kappock
• Publication: A multisubunit Gram-negative FGAM synthetase (unpublished)

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 2710
• Total vector size (bp): 3092
• Modifications to backbone: removed NdeI (vector) - NdeI (insert) segment, deleting 150 bp of vector (including lacZ promoter and M13-forward primer binding site)
• Vector type: Gene synthesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: formylglycinamidine ribonucleotide synthetase, small subunit
• Alt name: purS
• Alt name: AZ09_06375
• Species: Acetobacter aceti 1023
• Mutation: synthetic gene
• GenBank ID: KDE20458
• Promoter: n/a

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: none (AmpR)
• 3′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: hypothetical protein in purSQL operon
• Alt name: OrfY
• Alt name: AZ09_06380
• Species: Acetobacter aceti 1023
• Mutation: synthetic gene
• GenBank ID: KDE20459
• Promoter: none
• Tag / Fusion Protein:
  • His6 (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: M13-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The region 3' to the orfY gene includes partial sequence of purQ needed for cassette cloning.